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ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14

Issue per Year : 12

Volume Published : 11

Issue Published : 118

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Paper Title: Amino acid analysis of soybean based synbiotic product by derivatization free high performance liquid chromatography
Authors Name: Ansari Shafreen Gulam Mustafa , Dr. Ashish Asrondkar , Prof. R.J. Janephalkar , Dr. Vrushali Patil
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IJRTI_182587
Published Paper Id: IJRTi2206157
Published In: Volume 7 Issue 6, June-2022
DOI:
Abstract: For the purpose of preparing synbiotic functional food product, Liquid state fermentation of soybean and beetroot (prebiotic) extract was performed using Lactobacillus casei, Bacillus subtilis and Saccharomyces blaurdii. This study aimed to evaluate the changes in amino acid content of whole soybean and synbiotic soy-based product on 48 hr of fermentation maintained at 37ºC aerobically. The amino acid analysis was performed using Derivatization free RP-HPLC (Reverse Phase- High Performance Liquid Chromatography) method with detector as DAD (Diode Array Detector) at 262nm. The results obtained showed enhancement in essential amino acid content in Soy-based synbiotic liquid than whole soybean. Fermentation of Soybean shown increase in free amino acids specifically levels of Glutamic acid, lysine, Leucine and Isoleucine. Present research work implied that there is potential to develop nutritious soy-based synbiotic product, which will be a better approach to non-diary probiotics for lactose and gluten intolerant population.
Keywords: Synbiotic, amino acids, RP-HPLC, Fermentation, Lactobacillus casei, Bacillus subtilis, Saccharomyces blaurdii
Cite Article: "Amino acid analysis of soybean based synbiotic product by derivatization free high performance liquid chromatography ", International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2455-2631, Vol.7, Issue 6, page no.969 - 973, June-2022, Available :http://www.ijrti.org/papers/IJRTi2206157.pdf
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ISSN: 2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publication Details: Published Paper ID: IJRTi2206157
Registration ID:182587
Published In: Volume 7 Issue 6, June-2022
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Page No: 969 - 973
Country: thane, maharashtra, India
Research Area: Life Sciences 
Publisher : IJ Publication
Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTi2206157
Published Paper PDF: https://www.ijrti.org/papers/IJRTi2206157
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED, Journal Starting Year (ESTD) : 2016

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