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International Journal for Research Trends and Innovation
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ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Issue: June 2026
Volume 11 | Issue 6
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Impact Factor : 8.14
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| Paper Title: | Production and partial characterization of peroxidase enzyme from the general plant body and callus cultures of Piper nigrum L. and its application in bioremediation |
| Authors Name: | Nisha Rani D |
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| Author Reg. ID: |
IJRTI_212606
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| Published Paper Id: | IJRTI2605093 |
| Published In: | Volume 11 Issue 5, May-2026 |
| DOI: | |
| Abstract: | Peroxidases are oxidoreductase enzymes with significant potential for the bioremediation of synthetic dyes from industrial wastewater. This study investigates the production, partial characterization, and dye degradation efficiency of peroxidase enzyme extracted from the leaves and callus cultures of Piper nigrum L. (black pepper), a locally abundant plant in Kerala, India. The enzyme was partially purified using ammonium sulfate precipitation (50–75%), dialysis, and ultrafiltration (10 kDa NMWCO), resulting in a specific activity of 235 U/mg. The partially purified peroxidase exhibited optimal activity at pH 4.5–7.0 and temperature range of 35–50°C, with good thermal stability at 35°C. For enhanced enzyme production, callus cultures were initiated from leaf and stem explants on Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-D and BA. The optimal callus induction (green friable callus) was achieved with 2.0 mg/L 2,4-D and 1.5 mg/L BA. However, callus proliferation was accompanied by phenolic exudation and browning, which surprisingly showed tremendous peroxidase activity. Supplementation with 1% (w/v) polyvinyl pyrrolidone (PVP) reduced browning but simultaneously suppressed enzyme activity. The crude enzyme extract was effectively employed for the degradation of crystal violet (gentian violet), a recalcitrant triarylmethane dye. Almost complete degradation was achieved at dye concentrations up to 7.5 mg/L under optimal conditions. Degradation was confirmed by UV-Vis spectrophotometry (λmax 585 nm), TLC, and HPLC analysis. The findings demonstrate that Piper nigrum leaf peroxidase is a cost-effective, eco-friendly alternative to commercial horseradish peroxidase for dye bioremediation. Furthermore, callus cultures showing phenolic-associated enzyme activity present a promising avenue for developing reusable immobilized biocatalyst systems for industrial wastewater treatment. |
| Keywords: | Bioremediation, callus culture, dye degradation, peroxidase, Piper nigrum, phenolic exudation, plant tissue culture, triarylmethane dye. |
| Cite Article: | "Production and partial characterization of peroxidase enzyme from the general plant body and callus cultures of Piper nigrum L. and its application in bioremediation", International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2456-3315, Vol.11, Issue 5, page no.a753-a757, May-2026, Available :http://www.ijrti.org/papers/IJRTI2605093.pdf |
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| ISSN: |
2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID: IJRTI2605093
Registration ID:212606
Published In: Volume 11 Issue 5, May-2026
DOI (Digital Object Identifier):
Page No: a753-a757 Country: Thiruvananthapuram, Kerala, India Research Area: Life Sciences Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2605093 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2605093 |
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
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