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International Journal for Research Trends and Innovation
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14
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| Paper Title: | Study of the effect of UV-B for short duration on the callus culture of Bacopa monnieri by its qualitative and quantitative analysis using Thin layer chromatography and High-performance liquid chromatography |
| Authors Name: | Sonali Rajput , Sonali Rajput |
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IJRTI_185904
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| Published Paper Id: | IJRTI2304052 |
| Published In: | Volume 8 Issue 4, April-2023 |
| DOI: | |
| Abstract: | In this study an attempt was made to study the effect of UV-B for short duration on the callus culture of Bacopa monnieri by its qualitative and quantitative analysis using Thin layer chromatography and High performance liquid chromatography . Bacopa monnieri L.Pennell is an important medicinal plant Which belongs to family Plantaginaceae It is rich in innumerable bioactive secondary metabolites especially which are used as neuro tonic and treatment for depression, mental health and to increase cognitive performance . Five different callus culture groups were made on the basis of different concentrations of PGRs and each group had both non treated and UV treated sets in both the sets MS media was supplemented with different concentrations of PGRs. The non treated groups were maintained for 8 weeks without UV-B exposure but in the treated groups the cultures were maintained for 7 weeks and in the 8th week UV-B radiation were given for a period of 2hours/day Among all the 5 groups the best response were found in the group in which MS media was supplemented with 0.1mg/L of 2,4-D and BAP so these sets were taken for phytochemical studies . According to the present studies it was found that tissue culture and UV-B supplementation for short duration enhanced the secondary metabolite Bacoside which is a triterpenoid saponin and is responsible for the nootropic activity of the plant .When both the groups were compared the UV treated groups showed increased number of peaks as well as area in HPLC analysis and high range of saponins were detected in TLC analysis of UV treated callus extracts. |
| Keywords: | Bacopa monnieri ,HPLC,TLC,UV-B |
| Cite Article: | "Study of the effect of UV-B for short duration on the callus culture of Bacopa monnieri by its qualitative and quantitative analysis using Thin layer chromatography and High-performance liquid chromatography", International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2455-2631, Vol.8, Issue 4, page no.310 - 315, April-2023, Available :http://www.ijrti.org/papers/IJRTI2304052.pdf |
| Downloads: | 000205187 |
| ISSN: |
2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID: IJRTI2304052
Registration ID:185904
Published In: Volume 8 Issue 4, April-2023
DOI (Digital Object Identifier):
Page No: 310 - 315 Country: Dewas , Madhya Pradesh, India Research Area: Biological Science Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2304052 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2304052 |
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
Journal Starting Year (ESTD) : 2016
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