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International Journal for Research Trends and Innovation
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14
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| Paper Title: | Evaluate the efficacy of real time pcr for MBL detection in phenotypically confirmed clinical isolates of pseudomonas aeruginosa in various clinical samples in a Tertiary Care Hospital |
| Authors Name: | Dr. Shah Harshada , Chauhan Prasad Surendra , Md. Mustafa Sofiur Rahman |
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IJRTI_182348
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| Published Paper Id: | IJRTI2206236 |
| Published In: | Volume 7 Issue 6, June-2022 |
| DOI: | |
| Abstract: | CONTEXT: Carbapenems are the primary choice of treatment for severe Pseudomonas aeruginosa infection. However, the emergence of carbapenem resistance due to the production of Metallo-β-lactamases (MBLs) is of global concern. MATERIALS AND METHODS: A total 200 clinical isolates of Pseudomonas aeruginosa were tested for MBL production. Meropenem (MRP) resistant Pseudomonas aeruginosa were taken as MBL screening. MBL detection was done by three phenotypic methods 1) Combined disk synergy test (CDST). 2) Double disk synergy test (DDST) and 3) E-test. The real time pcr test was used for detection of two gene encoding MBLs (IMP & VIM). RESULTS: out of 200 Pseudomonas aeruginosa, 73 were resistant to Meropenem as screening positive. Out 73, 34(46.5%) isolates were MBL positive by CDST methods, 33(45%) by DDST method and 33(45%) by E-test. All 33 E-test positive strains contained the MBL gene where blaVIM and blaIMP gene were 23 and 10 respectively. CONCLUSION: The study show that the high prevalence of Pseudomonas aeruginosa producing MBL enzymes thus the proper antibiotics policy and all carbapenems resistant P. aeruginosa should be routinely screened for MBL production using phenotypic methods and that Real time-PCR confirmation be performed to minimize the emergence of this MBL producing pathogens. |
| Keywords: | Pseudomonas aeruginosa, Metallo-β-lactamase, CDST, DDST and E-test, blaVIM and blaIMP gene. |
| Cite Article: | "Evaluate the efficacy of real time pcr for MBL detection in phenotypically confirmed clinical isolates of pseudomonas aeruginosa in various clinical samples in a Tertiary Care Hospital", International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2455-2631, Vol.7, Issue 6, page no.1596 - 1601, June-2022, Available :http://www.ijrti.org/papers/IJRTI2206236.pdf |
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2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID: IJRTI2206236
Registration ID:182348
Published In: Volume 7 Issue 6, June-2022
DOI (Digital Object Identifier):
Page No: 1596 - 1601 Country: -, -, - Research Area: Engineering Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2206236 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2206236 |
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
Journal Starting Year (ESTD) : 2016
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