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Paper Title
Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture
Authors
Riya Patel , Riddhi Prajapati , Bhumika Patel , Nehal Nayak , Dr. Ravi R Patel
Keywords
Amlodipine, Metoprolol, telmisartan, RP-HPLC, validation, ICH
Abstract
A novel, simple, rapid, precise and highly efficient, isocratic liquid chromatography (RP-HPLC) method has been developed and validated for simultaneous estimation of amlodipine, metoprolol and telmisartan. Separation of all three compounds was achieved using Cosmosil C18 (250x4.6), 5µ column set at 25 °C temperature. Mobile phase consisted of a mixture of 20mMol KH2PO4: Acetonitrile:Methanol (30:30:40)
%v/v flowing at a flow rate of 1.5 mL/min. Detection was carried out by UV detector set at 234 nm. Injection volume was kept at 5 µL. The retention times were found to be 3.913 min, 2.107, 8.859 minutes for amlodipine, metoprolol and telmisartan respectively. The calibration curve demonstrated linearity over the range of 25-75 µg/mL, 250-750 µg/mL, 200-600 µg/mL for amlodipine, metoprolol and telmisartan respectively with correlation coefficients of 0.999 for all three drugs. The recoveries were found to be in the ranges of 100.1-100.4%, 100.1-100.8%, 99.2-100.0% for amlodipine, metoprolol and telmisartan respectively. The method was found to be precise and robust with very less %RSD values. The method can be employed for routine quality control analysis.
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IJRTI — journal style
"Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture", IJRTI - International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2456-3315, Vol.10, Issue 9, page no.a1-a23, September-2025, Available :https://ijrti.org/papers/IJRTI2509001.pdf
APA — 7th edition
Patel, R., Prajapati, R., Patel, B., Nayak, N., & Patel, R. R. (2025). Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture. International Journal for Research Trends and Innovation, 10(9), a1-a23. https://ijrti.org/viewpaperforall.php?paper=IJRTI2509001
MLA — 9th edition
Patel, Riya, et al. "Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture." International Journal for Research Trends and Innovation, vol. 10, no. 9, 2025, pp. a1-a23, https://ijrti.org/viewpaperforall.php?paper=IJRTI2509001.
Chicago — 17th, bibliography
Patel, Riya, et al. "Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture." International Journal for Research Trends and Innovation 10, no. 9 (2025): a1-a23. https://ijrti.org/viewpaperforall.php?paper=IJRTI2509001.
Harvard — author–date
Patel, R. et al. (2025) 'Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture', International Journal for Research Trends and Innovation, 10(9), pp. a1-a23. Available at: https://ijrti.org/viewpaperforall.php?paper=IJRTI2509001
IEEE — numbered reference
R. Patel, R. Prajapati, B. Patel, N. Nayak and R. R. Patel, "Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture," IJRTI, vol. 10, no. 9, pp. a1-a23, Sep. 2025.
Vancouver — biomedical
Patel R, Prajapati R, Patel B, Nayak N, Patel RR. Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture. IJRTI. 2025 Sep;10(9):a1-a23.
AMA — 11th edition
Patel R, Prajapati R, Patel B, Nayak N, Patel RR. Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture. IJRTI. 2025;10(9):a1-a23. Accessed at https://ijrti.org/viewpaperforall.php?paper=IJRTI2509001
BibTeX — Zotero, Mendeley, LaTeX
@article{IJRTI2509001,
author = {Riya Patel and Riddhi Prajapati and Bhumika Patel and Nehal Nayak and Ravi R Patel},
title = {Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture},
journal = {International Journal for Research Trends and Innovation},
volume = {10},
number = {9},
pages = {a1-a23},
year = {2025},
month = {September},
issn = {2456-3315},
url = {https://ijrti.org/viewpaperforall.php?paper=IJRTI2509001}
}
RIS — EndNote, RefWorks
TY - JOUR
AU - Patel, Riya
AU - Prajapati, Riddhi
AU - Patel, Bhumika
AU - Nayak, Nehal
AU - Patel, Ravi R
TI - Development and Validation of RP-HPLC Method for Simultaneous Determination of Metoprolol Succinate, Telmisartan, and Amlodipine Besylate in Synthetic Mixture
T2 - International Journal for Research Trends and Innovation
JA - IJRTI
VL - 10
IS - 9
PY - 2025
SN - 2456-3315
UR - https://ijrti.org/viewpaperforall.php?paper=IJRTI2509001
SP - a1-a23
ER -
Issue
Volume 10 Issue 9,
September-2025
Pages : a1-a23
Other Publication Details
Paper Reg. ID
IJRTI_205774
Published Paper ID
IJRTI2509001
Downloads
205,659
Research Area
Pharmacy
Country
Gandhinagar , Gujarat, India
Published
September 2025
Published Paper PDF
https://ijrti.org/papers/IJRTI2509001.pdf
Published Paper URL
https://ijrti.org/viewpaperforall.php?paper=IJRTI2509001
About Publisher
International Journal for Research Trends and Innovation
Published by IJRTI (JW Publication)
2456-3315
ISSN
10.57
Impact Factor
2016
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Funding
No external funding was received for this study.
Conflict of Interest
The authors declare that they have no conflict of interest.
Acknowledgements
The authors would like to thank the reviewers and the editorial board of International Journal for Research Trends and Innovation for their careful reading and constructive comments, and all colleagues who supported the preparation of this manuscript.
