Correlation of Vitamin D3 and Serum Calcium with HbA1c in Type 2 Diabetes Mellitus: A Cross-sectional Study

Sarveshwaraiah J , Dipti singh , Ananta Krishna Avinash B , Satish Kumar K , Sunil Kumar

Keywords: Vitamin D3 and Serum Calcium, HbA1c and Type 2 Diabetes Mellitus

Background: The role of vitamin D3 and serum calcium in T2DM remains a very important aspect which needs further evaluation to find out whether a correlation exist or not. So far, limited structured studies have been carried out on correlation of HbA1c with vitamin D3 and serum calcium level, hence, a cross sectional study was carried out to determine the same and establish a correlation. AIM: To correlate between serum vitamin D3 and serum calcium levels with HbA1c in newly diagnosed type 2 diabetes mellitus patients OBJECTIVES: The specific objectives of the studies were as follows: 1. To measure serum vitamin D3, serum calcium and HbA1c in newly diagnosed type 2 diabetes mellitus patients 2. To correlate serum vitamin D3 and serum calcium with HbA1c in newly diagnosed type 2 diabetes mellitus patients Methodology: : This cross sectional study was carried out at the Medicine Department of Rohilkhand Medical College and Hospital in Bareilly, Uttar Pradesh After the approval of the Institutional Ethics Committee (IEC), informed consent was taken from all the subjects participating and then the history was taken, clinical examination carried out and investigations were sent to the laboratory. Under all aseptic precautions, 5 ml venous blood was withdrawn and collected in red capped plain vacutainer, purple capped EDTA vacutainer and grey capped NaF vacutainer and sent for analysis to the laboratory for HbA1c, serum vitamin D3, serum calcium, fasting plasma glucose and post prandial plasma glucose levels. HbA1c was tested by BEACON Kit on Auto analyzer EM360 machine. Vitamin D3 level was tested by Chemiluminescence Immunoassay on Beckman Coulter Access 2 Machine. Fasting plasma glucose and post prandial plasma glucose levels were tested by Erba Mannheim Glucose reagent on Auto analyzer EM360 machine. Serum calcium level was tested by AUTOSPAN Liquid Gold Calcium (O- Cresolpthalein complex) on Auto analyzer EM360 machine. Results: 82.35% of people having HbA1c <7% have vitamin D3 levels <30 ng/dL whereas 17.65% of people having HbA1c <7% have vitamin D3 levels >30 ng/dL. People having HbA1c ≥7% have <30 ng/dL vitamin D3 levels in 68.49% and >30 ng/dL in 31.51%. The following data with p value 0.046 indicates statistically significant correlation. CONCLUSION: The correlation of HbA1c with 25(OH)D levels in newly diagnosed T2DM was statistically significant with p value < 0.05 (=0.046), though there was no inverse linear correlation found. The patients with HbA1c <7% (82.35%) and ≥7% (68.49%) had <30 ng/dL vitamin D3 level.  Vitamin D3 and calcium levels showed an inverse relation with each other, being statistically significant with p-value < 0.05. 

Volume 9 Issue 8, August-2024

Pages : 268 - 274