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Paper Title: REVIEW ON CRITICAL PROCESS PARAMETER FOR SELECTION OF MOBILE PHASE FOR HPLC
Authors Name: Dube Dnyaneshwari Suresh , Kolhe Nayan Tulashiram , Dube Vaishnavi Suresh , Mahesh Deshpande , A. T. Varungase
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IJRTI_189825
Published Paper Id: IJRTI2405035
Published In: Volume 9 Issue 5, May-2024
DOI:
Abstract: In HPLC (High-Performance Liquid Chromatography), the mobile phase comprises either pure or mixed diluters, sometimes through solid modifiers. Chromatographers have a plethora of diluters options for various HPLC applications. The selection process is influenced by diluters properties such as viscosity, refractive index, miscibility, toxicity, transparency, nonlethal and more. Obtainability in the industry at a realistic cost and high purity is also crucial. The diluters composition, particularly the percentage of carbon-based diluters in the moveable phase, directly impacts the retention time (tR) of analytes. A general guideline in Reversed-Phase Liquid Chromatography (RPLC) suggests that a 10% reduction in carbon-based diluters content leads to roughly a threefold increase in retention time. When separating acidic or basic samples, it's highly recommended to adjust the moveable phase pH by addition of a buffer. Selecting a suitable buffer involves considerations such as buffer capacity, solubility, interaction with samples or columns, and the risk of corrosion to HPLC equipment. Typically, a buffer concentration ranging from 10 to 50 millimeter suffices for utmost reversed-phase applications. During mobile phase preparation, diluters mixing involves measuring and combining each diluter sequentially in the reservoir. It's crucial to adjust the buffered moveable phase pH before addition of carbon-based diluters to minimize ambiguity regarding the final pH due to diluters addition. While this method introduces some uncertainty, it's preferable to the potential reproducibility issues associated with measuring pH after carbon-based diluters addition. Aqueous moveable phases comprising buffers should undergo filtration through a 0.2 micrometer membrane filter and degassing via vacuum filtration or sonication.
Keywords: HPLC, Solvent system,mobile phase
Cite Article: "REVIEW ON CRITICAL PROCESS PARAMETER FOR SELECTION OF MOBILE PHASE FOR HPLC", International Journal of Science & Engineering Development Research (www.ijrti.org), ISSN:2455-2631, Vol.9, Issue 5, page no.239 - 243, May-2024, Available :http://www.ijrti.org/papers/IJRTI2405035.pdf
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ISSN: 2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publication Details: Published Paper ID: IJRTI2405035
Registration ID:189825
Published In: Volume 9 Issue 5, May-2024
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Page No: 239 - 243
Country: Ahemednagar, MAHARASHTRA, India
Research Area: Pharmacy
Publisher : IJ Publication
Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2405035
Published Paper PDF: https://www.ijrti.org/papers/IJRTI2405035
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED, Journal Starting Year (ESTD) : 2016

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